Detection of antibodies anti-gp43 of Paracoccidioides brasiliensis in sera samples by double-sandwich ELISA

Luís Felipe Minozzo Figueiredo; Barbara Ribeiro Fonseca-de-Souza; Alessandra Becker-Finco; Elizângela Almeida Rocha; Luísa Lasmar Lima Oliveira; Ricardo Machado-de-Ávila; João Carlos Minozzo; Fernanda Costal-Oliveira; Larissa Magalhães Alvarenga; Vanete Soccol; Carlos Chávez-Olórtegui*

    Monoclonal antibodies (mAbs) were raised against 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis. One mAb anti-gp43 and gp43 were used in a double-antibody sandwich ELISA to detect antibodies in sera from patients with Paracoccidioidomycosis. This ELISA diagnosis showed 91.18% sensibility and 100.0% specificity against sera of patients with histoplasmosis, aspergillosis or chromomycosis.

Microbial load in unpasteurized milk obtained from selected milk outlets in Kawempe division of Kampala, Uganda

John N Kateregga; Collins Atuheire; Esther Nabatta; Samuel Majalija; James G Ndukui*

    Background: Milk consumption and demand has increased drastically over the past years in response to expanding human population in Uganda and world at large. Despite this, many residents of Kampala city obtain milk from shops supplied by milk vendors, who in turn, collect it directly from farms. The microbial load of this unpasteurized milk is not known, yet it could expose the population to milk-borne diseases..

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