Detection of antibodies anti-gp43 of Paracoccidioides brasiliensis in sera samples by double-sandwich ELISA

Luís Felipe Minozzo Figueiredo; Barbara Ribeiro Fonseca-de-Souza; Alessandra Becker-Finco; Elizângela Almeida Rocha; Luísa Lasmar Lima Oliveira; Ricardo Machado-de-Ávila; João Carlos Minozzo; Fernanda Costal-Oliveira; Larissa Magalhães Alvarenga; Vanete Soccol; Carlos Chávez-Olórtegui*

    Monoclonal antibodies (mAbs) were raised against 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis. One mAb anti-gp43 and gp43 were used in a double-antibody sandwich ELISA to detect antibodies in sera from patients with Paracoccidioidomycosis. This ELISA diagnosis showed 91.18% sensibility and 100.0% specificity against sera of patients with histoplasmosis, aspergillosis or chromomycosis.

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